Microbiology

Prepare Liver infusion media with Thermo Scientific™ Oxoid™ Liver Desiccated Bacteriological. Liver Desiccated is prepared by the dehydration of fresh pork livers under carefully controlled conditions designed to ensure maximum retention of nutritive properties, and is equivalent to five times its weight of fresh liver.

• Dehydrated whole liver, specially manufactured for the preparation of infusion media.
• Not all products are available for sale in all territories. Please inquire.
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Oxoid Streptococcus Supplement is used for the isolation of Streptococci.

• Add to Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Streptococcus Selective Supplement, Oxoid Composition

Streptococcus Selective Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500ml of sterile Columbia Blood Agar Base containing 5% Defibrinated Horse Blood SR0050 cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Enrich Enterobacteriaceae in bacteriological examination of foods and animal feed stuffs with Thermo Scientific™ Oxoid™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated). This medium is more inhibitory to non-Enterobacteriaceae than other non-selective media e.g. Mannitol broth or Lactose broth by virtue of the presence of brilliant green and bile salts in the preparation.

EE Broth, Oxoid Composition

EE Broth, Oxoid Preparation:
Add 43.5g to 1 litre of distilled water. Distribute 100ml quantities in 250ml flasks and heat at 100°C for 30 minutes only. Cool rapidly in cold running water. This medium is heat sensitive. DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Avoid overheating the medium, especially the double-strength broth.

Oxoid MRS Agar (de Man, Rogosa, Sharpe) (Dehydrated) is used for the growth of Lactobacilli.

Composition of MRS Agar, Oxoid

Preparation of MRS Agar, Oxoid

Suspend 62g of your MRS Agar in 1 litre of distilled water. You can boil to dissolve the powder completely. After a dissolve, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Conditions and Shelf Life of MRS Agar, Oxoid

You can store your dehydrated MRS Agar at 10-30°C and use before the expiry date on the label. You can store the prepared plates at 2-8°C.

Brilliance Candida is a chromogenic medium for the isolation and differentiation of Candida species.

• Add to to Brilliance Candida Agar Base, Part No. CM1002A and CM1002B
• Each vial supplements 500mL of medium

Brilliance Candida Selective Supplement, Oxoid Composition 

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Selective Supplement must be stored at 2-8°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Vitox Supplement is used for the preparation of Thayer Martin Medium and New York City Medium for the isolation of Neisseria spp.

• Contents: Vitox Supplement (5 vials) plus reconstitution fluid (5 vials)
• Add to GC Agar Base, Part No. CM0367B

Ordering Information:

• Each vial of Vitox plus reconstitution fluid supplements 500mL of medium

Includes:

• Five vials of Vitox plus five vials of reconstitution fluid

Vitox Supplement, Oxoid Composition

Vitox Rehydration Fluid , Oxoid Composition

Vitox, Oxoid Preparation:
Aseptically add one vial of Hydration Fluid to one vial of Vitox. Mix gently to dissolve completely. Aseptically add the contents of one vial to sterile medium of choice, cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid Yersinia Selective Supplement is used for the isolation of Yersinia enterocolitica.

• Add to Yersinia Selective Agar Base, Part No. CM0653B
• Each vial supplements 500mL of medium

Yersinia Selective Supplement, Oxoid Composition

Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.

Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.

Perform effortless Antimicrobial Susceptibility Testing (AST) using Thermo Scientific™ Oxoid™ Mueller Hinton Agar (Dehydrated), a medium designed and manufactured according to the European standard, EUCAST and the American standard, CLSI.

Composition of Mueller Hinton Agar, Oxoid

Preparation of Mueller Hinton Agar, Oxoid
Add 38g of Mueller Hinton Agar to 1 litre of distilled water. Bring to the boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life:
Store the dehydrated Mueller Hinton Agar, Oxoid at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton Agar plates at 2-8°C.

Precautions
Incubation in a carbon dioxide enriched atmosphere is not recommended because of its pH effect on the medium. If it is imperative to use CO₂ then known control organisms should be included with the test plates to measure its effect.
Carbohydrates should not be added to Mueller-Hinton Agar because they may influence the rate of growth of the organism and the resulting pH of the medium.
The addition of lysed horse blood to the medium may further reduce the levels of thymidine and prevent the growth of thymidine-dependent organisms.

The medium may not support the growth of some fastidious strains for example microorganisms that require thymine and thymidine for growth. Close adherence to the methodology is required to obtain satisfactory results.

Use Thermo Scientific™ Oxoid™ Sodium Biselenite in preparation of Selenite Broth Base (CM0395), Mannitol Selenite Broth Base (CM0399) or Selenite Cystine Broth Base (CM0699).  The complete medium is recommended for selective enrichment of Salmonella species from various samples.

• Dissolve 4 grams in 1 liter of distilled water and use this solution to reconstitute the base medium or tablets.
• Toxic by inhalation and if swallowed. Danger of cumulative effects.
• Not all products are available for sale in all territories. Please inquire.
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

• Oxoid TTC Solution (0.125%) is used for the enumeration and identification of coliform bacteria.
• Add to Tergitol 7 Agar, Part No. CM0793B.
• Each vial for 100mL medium.

Baird Parker Agar Base, Oxoid is suitable for the isolation and enumeration of Staphylococcus aureus, with high selectivity and sensitivity. It contains carefully balanced selective inhibitory agents, glycine, lithium, and tellurite, to suppress the growth of most of the bacteria present in foods without inhibiting coagulase-positive staphylococci. The presence of egg yolk emulsion and sodium pyruvate aid the recovery of damaged cells for presumptive identification of Staphylococcus aureus in food specimens.

Baird Parker Agar Base, Oxoid Preparation:

Suspend 6.3g of Baird-Parker Agar Base (RPF) in 90ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121ºC for 15 minutes. Cool to 48ºC and add 1 vial of RPF Supplement (SR0122A), reconstituted as directed. Mix well and pour plates.

Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and RPF Supplement below 0°C. Use before the expiry date on the label.
Prepared plates of the medium are best used freshly prepared.

Precautions
Regard all suspicious colonies as Staphylococcus aureus regardless of negative reactions in the medium and carry out further tests.
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

This item is not available at the moment. Please refer to the alternative, Violet Red Bile Glucose Agar (CM0485B). However, please note that this product does not conform to ISO.

Selectively enumerate Enterobacteriaceae in food, animal feed, and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated) while adhering to ISO 21528 and ISO 11133:2014. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBGA (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBGA (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBGA (ISO) plates can be stored for up to 2 weeks when made from CM1082 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBGA (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBGA (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

• Oxoid Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium) is used for the detection of motile Salmonella spp. from food and environmental samples.
• Also available MSRV Selective Supplement, Part No. SR0161E and Novobiocin Supplement, Part No. SR0181E.

Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium), Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Precautions
The basal medium is very hygroscopic. When handling the powder a face mask and gloves must be worn, refer to the material safety data sheet.

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base while adhering to ISO 21527-1: 2008. Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar is specially designed to suppress the growth of spreading mold and bacteria while supporting the growth of species that cannot be isolated on Rose Bengal Chloramphenicol Agar or Acidified Potato Dextrose Agar.

Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Composition

Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Preparation:
Suspend 15.75g per 500ml (31.5g/l) of distilled water and heat to dissolve completely. Reconstitute Chloramphenicol Supplement (one vial SR0078E per 500ml or one vial SR0078H per 2 litres medium), as directed. Add the vial contents to the DRBC (ISO) Agar Base. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10–30°C and use before the expiry date.

Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Efficiently isolate and identify salmonellae from clinical, environmental and food samples with highly selective Thermo Scientific™ XLT-4 Agar. XLT-4 Agar contains sodium tetradecylsulfate which acts to inhibit many of the background flora organisms whose growth can cause problems with differentiation on other Salmonellae plating media. This enhanced selectivity allows a significant increase in the recovery of salmonellae, while reducing false-positive suspect colonies, saving time and reducing the costs of further identification of false-positive colonies.

XLT-4 Agar Base, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Schaedler Anaerobe Broth is a thioglycollate-free broth for the general growth of anaerobes.
• For use in blood cultures and antibiotic MIC studies.

Schaedler Anaerobe Broth, Oxoid Composition

Schaedler Anaerobe Broth, Oxoid Preparation:
Add 26.5g to 1 litre of distilled water and mix to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared broth in the dark at <15°C.

Precautions
As with all anaerobic broth media, it is important to avoid chemo-oxidation (overheating) and photo-oxidation (storage in the light) because such oxidative effects cause the inhibition of growth.

Selectively isolate Salmonella species from food samples with Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn) for food testing in compliance with ISO 6579:2002.

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Composition

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Preparation:
Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and bring to the boil. Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution prepared by dissolving 25g of potassium iodide in 10ml of water, adding 20g of iodine and then diluting to 100ml with sterile water. Also add the contents of four vials of Oxoid Novobiocin Selective Supplement SR0181 reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at room temperature. The prepared medium in its final form containing iodine/iodide solution + Novobiocin Selective Supplement SR0181 should be used immediately.

The Thermo Scientific™ Oxoid Tryptone ia a pancreatic digest of casein for use where high performance and uniformity of composition are of paramount importance.

Detect and enumerate Enterobacteriaceae in food products with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose Agar (Dehydrated). This glucose-containing selective medium gives reproducible counts of typical colonies for food safety analysis. In the examination of food stuffs, detection of the Enterobacteriaceae that ferment glucose to produce acid and/or gas has been recommended.

Violet Red Bile Glucose Agar (Dehydrated), Oxoid Composition

Violet Red Bile Glucose Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well and dispense into tubes or dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms may grow e.g. Aeromonas. The selective activity of this medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth. Medium for the pour-plate procedure should be freshly prepared, cooled to 47°C and used within 3 hours.

Oxoid MRVP Medium. A glucose-phosphate medium is used for the differentiation of the coli-aerogenes group by the Methyl Red and Voges-Proskauer tests.

MRVP Medium, Oxoid Composition

MRVP Medium, Oxoid Preparation:
Add 17g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-6°C.

Precautions
The MR-VP reactions are only part of the tests required to identify organisms.
Each laboratory should standardise on the inoculum density, volume of broth and the test container size.
MR tests require a minimum incubation of 48 hours before the pH indicator is added.
When using Barritt’s reagents add a-naphthol first and KOH second; do not reverse this order.
Vaughn et al. warned of false positive VP reactions if the completed tests are left standing for over an hour.

Oxoid Muller-Kauffmann Tetrathionate Broth Base is selective enrichment medium for isolation of Salmonella spp. and suppression of Proteus species.

Muller-Kauffmann Tetrathionate Broth Base, Oxoid Composition

Muller-Kauffmann Tetrathionate Broth, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil. Cool below 45°C and add, just prior to use, 19ml of iodine solution and 9.5ml of a 0.1% brilliant green solution. Mix well and fill out into sterile tubes or flasks.

Dissolve the potassium iodide in approximately 5ml of distilled water, add the iodide and gently warm the solution to completely dissolve it. Make up the volume to 100ml with distilled water.

Add the brilliant green to the distilled water and shake to dissolve the dye. Heat the solution to 100°C for 30 minutes and shake from time to time whilst cooling, to ensure that the dye has completely dissolved. Store in a brown glass bottle or away from light.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not autoclave the base broth.
Add the iodine solution and brilliant green just prior to use.
The medium is not suitable for the growth of Salmonella typhi, Salmonella sendai, Salmonella pullorum and Salmonella gallinarum.