Microbiology

Oxoid Listeria Selective Enrichment Supplement is used for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth, Part No. CM0862B
• Each vial supplements 500mL of medium

Listeria Selective Enrichment Supplement, Oxoid Composition

Listeria Selective Enrichment Medium Preparation:
Reconstitute one vial as directed, and add the contents to 500ml of  Listeria Enrichment Broth Base (CM0862). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before distributing into sterile containers in volumes as required.

Storage conditions and Shelf life
Listeria Selective Enrichment Supplement SR0141 as supplied should be stored at 2-8°C. When stored as directed the reagents are stable until the expiry date printed on the label.

Precautions
Listeria Selective Enrichment Supplement contains cycloheximide and is toxic if swallowed, inhaled or by skin contact. As a precaution when handling wear gloves and eye/face protection.
Acriflavine is activated by light and may form compounds inhibitory for Listeria.

Thermo Scientific™ Oxoid Lab-Lemco Powder is a Beef Extract made from specially selected raw materials.

• May be used in fermentation processes
• Industrial Reference: NMKL

• Oxoid EC Broth, Reduced Bile Salts is a selective enrichment broth for the growth of Escherichia coli O157 from food and environmental samples.
• Also available Novobiocin Selective Supplement, Part No. SR0181E.

EC Broth (Reduced Bile Salts), Oxoid Composition

EC Broth (Reduced Bile Salts), Oxoid Preparation:
Dissolve 18.3g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool the EC Broth (Reduced Bile Salts) to 50°C and aseptically add the contents of 1 vial of Novobiocin Supplement SR0181, reconsituted as directed. Dispense into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
When stored as directed, the supplement remains stable until the expiry date printed on the label.

• The Oxoid Baird-Parker RPF Agar Base is a selective medium for the isolation and enumeration of coagulase positive staphylococci from food.
• For use with RPF supplement, Part No. SR0122A
• Do not use Egg Yolk Tellurite Emulsion.

Baird-Parker Agar Base (RPF), Oxoid Composition

Baird-Parker Agar Base (RPF), Oxoid Preparation:
Suspend 6.3g of Baird-Parker Agar Base (RPF) in 90ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121ºC for 15 minutes. Cool to 48ºC and add 1 vial of SR0122A, reconstituted as directed. Mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and RPF Supplement below 0°C. Use before the expiry date on the label.
Prepared plates of medium are best used freshly prepared.

Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

Oxoid Rogosa Agar is used for the selective isolation and enumeration of lactobacilli.

Rogosa Agar, Oxoid Composition

Rogosa Agar, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil to dissolve completely. Add 1.32ml glacial acetic acid and mix thoroughly. Heat to 90-100°C for 2-3 minutes with frequent agitation. Distribute into sterile tubes, Petri dishes or bottles.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Lactobacillus carnis does not grow on this medium.
All colonies must be tested by Gram stain and catalase test before carrying out further identification tests.

Selectively enrich bile tolerant Gram-negative bacteria during microbiological examination of pharmaceutical products with Thermo Scientific™ Oxoid™ Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP).

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Composition

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Preparation:
Dissolve 25.97g in 1 litre of purified water. Mix well and distribute 100ml into 250ml flasks. Heat the medium at 100°C using free flowing steam for 30 minutes only. Cool rapidly in cold running water.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-25°C.

Oxoid Vogel-Johnson Agar is used for the detection of Staphylococcus aureus from foods and clinical specimens.

Vogel-Johnson Agar, Oxoid Composition

Vogel-Johnson Agar, Oxoid Preparation:
Suspend 61g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5.7ml of sterile 3.5% potassium tellurite solution SR0030 (equivalent to 20ml of 1% potassium tellurite).

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
All presumptive Staphylococcus aureus colonies should be confirmed with further tests. Do not heat the medium after the addition of potassium tellurite.

Oxoid Czapek Dox Agar Modified is used for cultivation of fungi.

Czapek Dox Agar Modified, Oxoid Composition

Czapek Dox Agar Modified, Oxoid Preparation:
Suspend 45.4g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. If required, adjust the reaction to pH 3.5 ± 0.2 by adding 10ml of Lactic Acid 10% (SR0021) per litre after sterilisation.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Salmonella Selective Supplement is used for the presumptive identification of Salmonella species from clinical and food samples.

• Add to Chromogenic Salmonella Agar Base, Part No. CM1007B or Brilliance™ Salmonella Agar Base, Part No. CM1092B
• Each vial supplements 500mL of medium

Salmonella Selective Supplement, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

• Oxoid Membrane Endo Agar LES (m Endo AgarLES) is an APHA recommended medium for the confirmation of coliforms.
• Also available Basic Fuchsin Indicator, Part No. BR0050A.

Membrane Endo Agar LES, Oxoid Composition

Membrane Endo Agar LES, Oxoid Preparation:
Suspend 45 grams in 1 litre of distilled water. Add 12ml of a 10% w/v solution of Basic Fuchsin. Heat gently with frequent agitation until the medium boils. DO NOT AUTOCLAVE. Cool to 45°C and dispense into 50-60 mm dishes in 4 ml volumes. For larger dishes use sufficient medium to give an equivalent depth (approx. 1.5 mm).
Plates should be protected from light and may be stored for up to two weeks in the refrigerator.

Storage conditions and Shelf life
Store the dehydrated medium below 25°C and use before the expiry date on the label.
Store the prepared medium in the dark and at 2-8°C.

Agar Bacteriological (Agar No. 1, Oxoid) is a clear purified high gel strength bacteriological agar compatible with all culture media. This agar has low calcium (Ca) and magnesium (Mg) levels and has very high working gel strength (1.5% w/v).

Agar Bacteriological, Oxoid is compatible with all culture media. It can be formulated to either broth or agar of the same medium with very similar metal values. This characteristic is especially valuable in antimicrobial MIC studies where differences in mineral/metal content can profoundly influence the results. Due to the low mineral/metal content in Agar Bacteriological, Oxoid, it allows free diffusion of antimicrobial substances. As a result, it is a highly satisfactory agar for antimicrobial diffusion studies (disc diffusion susceptibility tests).

Selectively isolate and differentiate Pseudomonas aeruginosa from a range of samples using Thermo Scientific™ Oxoid™ Cetrimide Agar (USP, EP) (Dehydrated). The medium contains magnesium chloride and potassium sulphate for improved pigment production and recovery of Pseudomonas aeruginosa while strongly suppressing Klebsiella, Proteus and Providencia spp. The medium conforms to European Pharmacopoeia for Microbial Limit Tests.

Cetrimide Agar (USP, EP) (Dehydrated), Oxoid Composition

Cetrimide Agar Preparation:
Suspend 45.3g of Pseudomonas Cetrimide Agar in 1 litre of distilled water. Add 10ml of glycerol and boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to approximately 50°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

• Oxoid Ampicillin Selective Supplement is used for the isolation of Aeromonas hydrophila.
• Add to to Aeromonas Medium Base, Part No. CM0833B.

Ampicillin Selective Supplement, Oxoid Preparation:

Suspend 29.5g in 500ml of distilled water. Bring gently to the boil. DO NOT AUTOCLAVE. Cool to 50°C and aseptically add one vial of Ampicillin Selective Supplement SR0136 reconsituted as directed. Mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium 2-8°C.

Enumerate heterotrophic bacteria from drinking water samples with Thermo Scientific™ Oxoid™ R2A Agar (Dehydrated). This nutritionally reduced medium was specially designed to enhance the recovery of stressed and chlorine damaged bacteria from treated water samples. Stressed or injured organisms during water treatment are unable to grow on high nutrient media since faster-growing organisms outgrow the former. Therefore the use of a low nutrient medium like R2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.

R2A Agar, Oxoid Composition

R2A Agar, Oxoid Preparation:
Suspend 18.1g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
R2A Agar should be stored tightly capped in the original container at 10-30°C. When stored as directed the medium will remain stable until the expiry date printed on the label.

The prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Remel™ Sputasol is a liquifying agent for sputum samples.

• Each vial dilutes in sterile distilled water to make 100mL

Sputasol, Oxoid Composition

Sputasol, Oxoid Preparation:
Aseptically add the contents of one vial (7.5ml) to 92.5ml of sterile distilled water. Use the working solution immediately or store at 2-8°C for upto 48 hours only.

Oxoid Urea Agar Base (Christensen Agar Base) is used for detection of urease-producing microorganisms.

Urea Agar Base, Oxoid Composition

Urea Agar Preparation:
Suspend 2.4g in 95ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C and aseptically introduce 5ml of sterile 40% Urea Solution SR0020. Mix well, distribute 10ml amounts into sterile containers and allow to set in the slope position.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The alkaline reaction produced in this medium after prolonged incubation may not be caused by urease activity. Check using medium without urea.
Do not heat or reheat the medium because urea decomposes very easily.
For the detection of urease-positive Proteae the reaction must be read within the first 2-5 hours of incubation.

Description

LB Agar Miller from Merck is a commonly used medium in microbiology laboratories and is suitable for routine bacterial culture and transformation experiments. It is a formulation of Luria-Bertani (LB) agar developed by Miller, which includes a mixture of peptides, amino acids, and vitamins to support the growth of a wide range of bacteria.

Selectively detect Escherichia coli from food and environmental samples with Thermo Scientific™ Oxoid™ EC Broth (Dehydrated). EC Broth is a selective medium for the differentiation of fecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples. It is also suitable for the enumeration of presumptive E. coli in milk and milk products using the most probable number technique.

EC Broth, Oxoid Composition

EC Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Dispense into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 4 weeks.

Oxoid Brilliant Green Agar (Kauffmann Medium) is used for increased recovery and selective isolation of Salmonella spp. other than S. Typhi.

• Also available Sulphamandelate Supplement, Part No. SR0087E

Brilliant Green Agar, Oxoid Composition

Brilliant Green Agar, Oxoid Preparation:
Suspend 50g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Lactose-fermenting salmonellae may be present in foods.
Salmonella typhi and Shigella species may not grow on this medium.
Proteus, Citrobacter and Pseudomonas species may mimic enteric pathogens by producing small red colonies.