Microbiology

Detect salmonellae including Salmonella arizonae with the diagnostic Thermo Scientific™ Oxoid™ Lysine Iron Agar (Dehydrated). This differential medium detects salmonellae (including lactose fermenting Salmonella arizonae) by lysine decarboxylase activity and H₂S production.

Lysine Iron Agar, Oxoid Composition

Lysine Iron Agar, Oxoid Preparation:
Suspend 34g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into tubes and sterilise by autoclaving at 121° C for 15 minutes. Cool the tubes in an inclined position to form slants with deep butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C.

Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.

Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.

MYP Agar, Oxoid Composition

MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thioglycollate Medium USP – Alternative, is intended for sterility testing with certain biological products that are turbid or otherwise do not lend themselves readily to culturing in Thioglycollate Medium USP (CM0173) because of its viscosity.

Thioglycollate Medium USP – Alternative, Oxoid Composition

Thioglycollate Medium USP – Alternative, Oxoid Preparation:
Suspend 29g in 1 litre of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilise by autoclaving at 121°C for 15 minutes.
PREPARE FRESHLY OR BOIL AND COOL THE MEDIUM JUST BEFORE USE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Whilst storage of the prepared medium is not recommended, autoclaved volumes of Thioglycollate Medium USP – Alternative, should be held at 20-30°C in the dark. Storage at lower temperatures increases oxygen absorption.

Precautions
This medium lacks agar and reducing indicator, therefore it is essential that the medium is freshly prepared and used within four hours of preparation.
Thioglycollate media should not be reheated more than once because toxic oxygen radicles are formed on reheating.

Isolate, enumerate and differentiate coliforms in dairy products with differential Thermo Scientific™ Oxoid™ Desoxycholate Agar (Dehydrated). The medium is also used for the isolation of enteric pathogens from rectal swabs, feces or other specimens. Differentiation of coliforms is based on the fermentation of lactose. Lactose fermenting bacteria form pink colored colonies while non-lactose fermenters form straw colored colonies.

Desoxycholate Agar, Oxoid Composition

Desoxycholate Agar, Oxoid Preparation:
Suspend 45g in 1 litre of distilled water. Bring to the boil over gauze and flame to dissolve the medium completely. Agitate to prevent charring.
THIS MEDIUM IS HEAT SENSITIVE. AVOID EXCESSIVE OR PROLONGED HEATING DURING RECONSTITUTION. DO NOT AUTOCLAVE OR REMELT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Kanamycin Sulphate Supplement (10mg/vial) is added to Kanamycin Esculin Azide Agar Base, Part No. CM0591B.

Kanamycin Sulphate Supplement, Oxoid Composition

Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Add 2ml of sterile distilled water to one vial and mix gently to dissolve completely. Add the contents to 500ml of Kanamycin Aesculin Azide Agar Base.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium
• Format: Freeze dried

Vancomycin Supplement, Oxoid Composition

Storage conditions and Shelf life
Vancomycin Supplement SR0186 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Isolate and enumerate lactic acid bacteria from food with Thermo Scientific™ Oxoid™ MRS (ISO) Agar (DeMan, Rogosa and Sharpe) (Dehydrated) while adhering to ISO 15214: 1998. This medium is designed to support the growth of all strains of Lactobacilli, even the difficult and slow-growing strains of Lactobacillus brevis and Lactobacillus fermenti.

MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Composition

MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Suspend 66.73g in 1 litre of distilled water. Boil to dissolve the medium completely. Dispense into tubes, bottles or flasks and sterilize by autoclaving at 121°C for 15 minutes. Selectivity can be altered by pH adjustment.

Storage conditions and Shelf life
Store the dehydrated medium at 10–30°C and use before the expiry date on the label.
Store the prepared plates at 2–8°C.

Precautions
Sterilization of this medium in some autoclaves, at 121°C for 15 minutes, may cause the medium in some cases to fall outside the specified pH limit of 5.7 ± 0.1. Adjustment of the medium in these rare cases is recommended using acetic acid or sodium hydroxide.

Isolate and identify Salmonella and Shigella species from clinical or food samples with Thermo Scientific™ Oxoid™ SS Agar (Dehydrated). Differentiation of Salmonella and Shigella is achieved by incorporation of lactose in the medium. Organisms which ferment lactose produce acid which, in the presence of the neutral red indicator, results in the formation of red colonies. Salmonella, Shigella, and other non-lactose-fermenting organisms appear as transparent or straw coloured colonies on SS Agar.

SS Agar, Oxoid Composition

SS Agar, Oxoid Preparation:
Suspend 63g in 1 litre of distilled water. Bring to the boil with frequent agitation and allow to simmer gently to dissolve the agar. DO NOT AUTOCLAVE. Cool to about 50°C, mix and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This medium is highly selective and R-strains of shigellae will not grow on it. It is not recommended for the primary isolation of shigellae.

Thermo Scientific™ Oxoid Soy Peptone is obtained by the hydrolysis of soya flour and complies with the USP specification.

Oxoid Peptone Bacteriological Neutralized is the neutralized form of Peptone Bacteriological.

Oxoid Tetrathionate Broth is used for the selective enrichment of Salmonella spp.

Tetrathionate Broth, Oxoid Composition

Tetrathionate Broth, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water and bring to the boil. Cool to below 45°C and add 20ml of iodine-iodide solution immediately before use. Mix continuously whilst dispensing 10ml volumes into sterile tubes. Use the complete medium (with added iodine) on the day of preparation.

The base may be prepared beforehand and kept for several weeks at 4°C. Iodine-iodide solution can then be added at the time of use to the quantity of medium needed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the base broth at 2-8°C. Use the medium immediately after adding the iodine solution.

Oxoid Buffered Listeria Enrichment is selective enrichment medium for the detection of Listeria monocytogenes.

Buffered Listeria Enrichment Broth, Oxoid Composition

Buffered Listeria Enrichment, Oxoid Preparation:
Add 23.5g to 500ml of distilled water and mix well to dissolve. Add the contents of 1 vial of Listeria Selective Enrichment Supplement SR0141 or the alternative Modified Listeria Selective Supplement SR0213 reconstitued as directed. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and aseptically distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Buffered Listeria Enrichment Broth should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored as directed the medium will remain stable until the expiry date printed on the label.

Thermo Scientific™ Oxoid Vegetable Peptone No. 1 is a highly nutritious, meat-free general purpose peptone for growth of bacteria and fungi.

Oxoid Campylobacter Growth Supplement is a liquid supplement for the enhanced growth and aerotolerance of Campylobacter species.

• For the preparation of Blaser-Wang Medium, Bolton Selective Enrichment Broth, Butzler Selective Medium, and Preston Selective Medium
• Each vial supplements 500mL of medium

Campylobacter Growth Supplement, Oxoid Composition

Campylobacter Growth Medium, Oxoid Preparation:
Add the contents of one vial (2 ml) to 500 ml of a sterile nutrient medium cooled to 50-55°C prepared from Oxoid Columbia Agar, Blood Agar Base No.2, or Campylobacter Agar Base, with 5-7% lysed defibrinated horse or sheep blood, and the rehydrated contents of one vial of Campylobacter Antibiotic Supplement SR0069, SR0085 or SR0098. Mix gently and pour aseptically into sterile Petri dishes.

Oxoid Nutrient Gelatin is used for the differentiation of microorganisms by their proteolytic effects.

Nutrient Gelatin, Oxoid Composition

Nutrient Gelatin, Oxoid Preparation:
Suspend 128g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring and cool below 20°C or leave to set in a refrigerator.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not shake the gelatin tubes whilst they are warm because growth and liquefaction of gelatin frequently occurs on the surface layer only.
In routine diagnostic work report gelatin liquefaction or not. The type or shape of liquefaction is of less importance.

Oxoid Urea Broth Base (Christensen Broth Base) is used for the detection of urease producing microorganisms.

Urea Broth Base, Oxoid Composition

Urea Broth Preparation:
Add 0.9 g to 95 ml of distilled water. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 55°C and aseptically introduce 5 ml of sterile 40% Urea Solution SR0020. Mix well and distribute 10 ml amounts into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is preferable that the medium be used on the day of preparation. If not, examine the tubes carefully to ensure sterility.
After overnight incubation other members of the Enterobacteriaceae may show alkaline reactions.

Oxoid Kligler Iron Agar is used for differential identificaton of Enterobacteriaceae.

Kligler Iron Agar, Oxoid Composition

Kligler Iron Agar, Oxoid Preparation:
Suspend 55g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set as slopes with 1 inch butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is essential that Kligler Iron Agar is examined and reported at 18-24 hours. Early or late readings will give false results.
KIA will grow both oxidative and fermentative organisms. Confusion will result if care is not taken to distinguish between the two groups.
Always use a straight wire to inoculate the butt, to avoid splitting the agar with a loop.
Pure cultures are essential to avoid misinterpretation.
Do not use screw-capped tubes or bottles for KIA medium. It is essential that air is freely available to growth on the slant.

Oxoid Bacillus Cereus Selective Supplement (Polymyxin B Supplement) is supplied 50,000 IU/Vial for inhibition of Gram-negative bacteria.

• May be added to Bacillus cereus Selective Agar Base, Part No. CM0617B and MYP Agar Base, Part No. CM0929B
• Each vial supplements 500mL of medium

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Composition

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Preparation:
Reconstitute as directed in the product instructions for use. Aseptically, add one vial to 500ml of liquid or molten base medium, which is below 50°C. Mix well, and dispense into appropriate, sterile containers.

Storage conditions and Shelf life
Polymixin B Supplement at 2-8°C and use before the expiry date on the label.

Selectively isolate Pseudomonas species from a range of samples with Thermo Scientific™ Oxoid™ Pseudomonas Agar Base (Dehydrated). The medium is modification of King’s A Medium and contains magnesium chloride and potassium sulphate for improved pigment production and recovery of Pseudomonas aeruginosa while strongly suppressing Klebsiella, Proteus and Providencia spp.

Pseudomonas Agar Base (Dehydrated), Oxoid Composition

Agar Preparation:
To Prepare the Agar Base:
Suspend 24.2g of the agar base, in 500ml of distilled water. Add 5ml of glycerol. Bring to the boil to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes. Allow the medium to cool to 50°C.

To Prepare Pseudomonas CN Agar:
To 500ml of agar base cooled to 50°C add the contents of 1 vial of Pseudomonas CN Supplement (SR0102) rehydrated as directed. Mix well and pour into sterile Petri dishes.

To Prepare Pseudomonas CFC Agar:
To 500ml of agar base cooled to 50°C add the contents of 1 vial of Pseudomonas CFC Supplement (SR0103) rehydrated as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Fresh media should be prepared as required. Molten agar should not be kept longer than 4 hours. Medium should not be stored and remelted. If swarming colonies of Proteus species are a problem in food samples then the incubation temperature can be lowered to 20°C for a period of 3-5 days. Chilled foods may carry a wide range of pseudomonads and the colonies on CFC Medium, incubated at lower temperatures, may be Pseudomonas fluorescens or Pseudomonas putida as well as Pseudomonas aeruginosa. Aeromonas species will also appear as pink/brown colonies, particularly from fish products.

Thermo Scientific™ Oxoid PALCAM Supplement is a selective supplement for use with PALCAM Agar Base.

PALCAM Supplement, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

MacConkey agar, Oxoid is used to detect, isolate and enumerate coliforms and intestinal pathogens in water, dairy products and biological specimens. This differential medium gives a number of diagnostic indications such as bile tolerance, colony morphology and chromogenesis. MacConkey Agar corresponds to the medium recommended by the World Health Organizaton, the Department of Health for the bacterial examination of water.

Composition of MacConkey Agar (Oxoid)

Preparation of MacConkey Agar (Oxoid):

Suspend 52g of MacConkey agar powder in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life of MacConkey Agar (Oxoid)
Store the dehydrated MacConkey agar powder at 10-30°C and use before the expiry date on the label.
Store the prepared MacConkey agar at 2-8°C.